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hs5 human bone marrow fibroblast cell line  (ATCC)


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    ATCC hs5 human bone marrow fibroblast cell line
    ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, <t>HS5.</t> Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.
    Hs5 Human Bone Marrow Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs5+human+bone+marrow+fibroblast+cell+line/pmc05292682-36-3-10?v=ATCC
    Average 97 stars, based on 317 article reviews
    hs5 human bone marrow fibroblast cell line - by Bioz Stars, 2026-07
    97/100 stars

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    1) Product Images from "Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma"

    Article Title: Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma

    Journal: Leukemia

    doi: 10.1038/leu.2016.187

    ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, HS5. Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.
    Figure Legend Snippet: ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, HS5. Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.

    Techniques Used: Knockdown, Expressing, Comparison, Over Expression, Mutagenesis



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    hs5 human bone marrow fibroblast cell line  (ATCC)
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    ATCC hs5 human bone marrow fibroblast cell line
    ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, <t>HS5.</t> Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.
    Hs5 Human Bone Marrow Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hs5+human+bone+marrow+fibroblast+cell+line/pmc05292682-36-3-10?v=ATCC
    Average 97 stars, based on 1 article reviews
    hs5 human bone marrow fibroblast cell line - by Bioz Stars, 2026-07
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    human bone marrow-derived stromal fibroblast cell lines hs5  (Pasteur Institute)
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    ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, <t>HS5.</t> Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.
    Human Bone Marrow Derived Stromal Fibroblast Cell Lines Hs5, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human bone marrow-derived stromal fibroblast cell lines hs5 - by Bioz Stars, 2026-07
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    ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, HS5. Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.

    Journal: Leukemia

    Article Title: Citrullination of histone H3 drives IL-6 production by bone marrow mesenchymal stem cells in MGUS and multiple myeloma

    doi: 10.1038/leu.2016.187

    Figure Lengend Snippet: ( a ) Confirmation of knockdown by siPADI2 by two independent siRNA sequences. ( b ) The expression of the BMMSC component of critical chemokine signalling axes in myeloma (IL-6, CXCL12 and cMET) is modulated by PADI2 expression in the immortalised BMMSC cell line, HS5. Expression of all three transcripts are reduced in response to reduction in PADI2 expression (siPADI2), or the addition of the pan-PADI inhibitor, Cl-Amidine (200 μ m , 72 h). However, siPADI4 does not alter expression of any of the transcripts. ** and *** indicate the level of significance assigned by a Dunn's comparison post-test after a significant ANOVA for each dataset ( P <0.0001 [IL-6] P =0.0002 [CXCL12] P =0.0019 [cMET]). ( c ) IL-6 expression, as measured through its secretion into the tissue culture medium by the HS5 cell line, is reduced after treatment with Cl-Amidine and/or siPADI2. Data shown are mean of n =3±s.d. * P =0.039, unpaired student's t -test with Welch's correction. ( d ) Overexpression of PADI2 increases IL-6 expression, whereas the catalytic mutant, C645S, does not increase expression over basal levels. Data shown are mean of n =3±s.d. ** P =0.004, unpaired student's t -test with Welch's correction.

    Article Snippet: BMMSCs and the HS5 human bone marrow fibroblast cell line (ATCC CRL11882, LGC Standards, Teddington, UK, purchased December 2013, tested for mycoplasma every 3 months by PCR) were cultured in RPMI1640 medium with 10% fetal bovine serum (Hyclone, GE Healthcare, Little Chalfont, UK) at 37 °C in a humidified incubator with 5% CO 2 .

    Techniques: Knockdown, Expressing, Comparison, Over Expression, Mutagenesis